Induced Pluripotent Stem Cells (iPSCs)
Since their initial demonstration in 2006 and subsequent implementation in human cells the following year[2,3], induced pluripotent stem cells (iPSCs) have ignited the possibility of regenerative medicine sourced from an adult patient’s own cells. However, challenges exist in translating these discoveries into therapies. Specifically, populations of stem cells that do not differentiate fully are tumorigenic in vivo[4,5] raising safety concerns. As a result, a need exists to further understand and analyze populations of stem cells as they differentiate. While several biochemical markers exist that could be used to measure the ability of cells to self-renew, expression levels often vary and significantly overlap between different cell lines as observed by flow cytometry. New fluorescently interrogated markers could be discovered that might aid in the specificity, but this adds additional cost and complexity to an already difficult task. Thus, a label-free method of analyzing these cells is desirable.
LumaCyte’s label free Radiance instrument has the potential to detect and sort cells based on subtle intrinsic differences, which could aid in the analysis of cell population composition and differentiation state. Differences have already been seen between two states of differentiation in a white blood cell line, as well as between two similar classes of white blood cells. Please contact us if you are interested in learning how our technology can be applied to help meet your iPSC research needs.