Laser Force CytologyTM
LumaCyte’s core product is Radiance, a Laser Force Cytology (LFC)TM instrument. This device uses a unique combination of advanced optics and microfluidics to automatically identify/characterize suspended cells and sort them into user definable classes, based upon their intrinsic properties. Users insert cells directly into the system without preparing a sample with a corresponding antibody label. The system then collects and analyzes data, sorts cell populations, and collects fractions of interest. In many applications, the cells of interest do not have known or available antibody labels or their use hampers the analysis or ruins the cells being studied. Radiance avoids these issues. The device also captures high resolution images of the cells passing through a laser beam, which are used to further identify cells for analysis and sorting. This combination of laser force measurements and imaging facilitates powerful analysis that is not possible using conventional label-based flow cytometry.
LumaCyte’s Radiance™ instrument uses optical forces to analyze and sort cells of interest without the use of labels. Optical forces arise when a sufficient number of photons from a laser reflect or refract through a cell, and are tightly correlated with changes in the intrinsic properties of the cells passing through the system. Since the instrument operates using intrinsic properties, antibody or genetic labels are not required, which frees the user to investigate changes in cell populations in an unbiased way. Radiance™ uses a laser wavelength that does not damage mammalian cells. LumaCyte’s microfluidic technology gently handles cells, resulting reduced cell damage when compared to other technologies. This allows for a greater percentage of viable cells that can be recovered, which can be especially important for sensitive cells or small samples.
Factors Affecting Optical Force
Differences in cell biochemistry and morphology (cytoskeletal changes), which are often associated with cancer, sepsis, and other diseases, give rise to detectable differences in optical force and deformability which are quantitated by Radiance™.