AAV Transfection Efficiency of Different Transfection Reagents
Transfection in gene therapy is generally used for the production of the viral vector intended to deliver the gene of interest into the patient. Typically, this process begins with the insertion of plasmids into the production cells via chemical means of opening the membrane temporarily to insert the plasmids. Chemical transfection is the most common method as it is simple and inexpensive to use compared with more sophisticated methods such as electroporation or microinjection. Most AAV therapies use chemical transfection in human embryonic kidney HEK 293 cells for the production of the indented AAV virus. Selecting the optimal transfection reagent and culture conditions is an important step for R&D and process development to ensure an efficient production process.
Additional applications of LFC™ throughout the AAV production process include adventitious agent monitoring to rapidly detect potential contamination as well as cell line characterization during process development and scale-up.