Rapidly Measuring Quantitative Early Indicators of Cellular Response to Viral Infection
A crucial measurement needed throughout vaccine development and manufacturing is the rapid quantification of viral infectivity or titer. Analytical monitoring of viral titer is important to help ensure predictable infection and expansion, maximum yield, and safety parameters, especially for cell cultured based processes. Variations in vaccine storage time, temperature, or formulation can also affect the number of infectious particles in a sample, necessitating a precise viral titer assay for potency. Traditional viral titering methods, such as plaque assays and TCID50, are time-consuming, labor-intensive, operator-dependent, and error-prone. These shortcomings lead to increased development time and cost, reduced formulation efficiency, need for over-formulation, shorter product shelf life, and difficulties in technology transfer across organizations and locations.