Developing More Efficient and Reproducible AAV-Based Gene Therapies Using Laser Force Cytology™
Adeno-Associated Virus (AAV) transduction is the process by which genetic material carried by AAV vectors is introduced into target cells. AAV is a commonly used viral vector in gene therapy and biotechnology due to its ability to efficiently deliver therapeutic genes into a variety of host cells without causing significant immune responses. During transduction, the AAV vector enters the target cell, and its genetic payload (transgene) is integrated into the host cell’s genome, leading to the expression of the desired gene. AAV transduction is crucial for various applications, especially in gene therapy, where it serves as a vehicle for delivering therapeutic genes to treat genetic disorders, certain cancers, and other diseases. It also plays a significant role in biotechnology for the production of recombinant proteins and in research for studying gene function and regulation.
Current challenges associated with AAV transduction include:
Optimizing efficiency across diverse cell types
Mitigating cellular heterogeneity to ensure consistent outcomes
Addressing time-dependent dynamics in transgene expression
Achieving standardized protocols for large-scale vector production
Navigating immune responses, such as neutralizing antibody formation
Laser Force Cytology™ (LFC) offers a powerful solution to enhance AAV transduction studies by providing detailed, label-free analysis. It enables the precise monitoring of transduction efficiency and dynamics, offering insights into cellular heterogeneity, facilitating the development of AAV-based gene therapies.
Improved AAV2 Analytics Using Laser Force Cytology™
LumaCyte’s Radiance® instrument and Laser Force Cytology™ technology allows for the precise and accurate measurement of AAV transduced cells without relying on helper viruses or specific cell lines, accelerating the production of life saving gene therapy products and improving the quality and consistency of key production processes.
