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Measurement of Infectious Titers and Total Viral Particle Counts to Fully Characterize Your Viral Process and Product

In this technical note, we demonstrate the impacts of various process insults, namely elevated temperature, low pH, and freeze-thaw cycles on the virulence of viral particles and the capability of Radiance® to assess these changes quickly. The Malvern Panalytical Nanosight Pro NS300 is also used for viral particle analysis prior to infection in order to better understand the viral starting material and how the ratio of total to infectious viral particles might change with various insults. The combination of these methods allows for the full characterization and function of a viral product. 

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