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Radiance® Label-Free Monitoring of AAV Transfection in HEK293 Cells Using Laser Force Cytology™ (LFC™)

In this technical note, collaborative efforts are described between Catalent and LumaCyte to compare AAV production with three different transfection reagents using both LFC™ and a digital droplet PCR (ddPCR) based viral genome titer assay. A strong correlation was generated between the LFC™ based measurements, which are available in real-time, and the ddPCR results, which take significant time and labor. This demonstrates the utility of LFC™ for rapid process monitoring of AAV transfection.

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